An Engineered Synthetic Pathway for Discovering Nonnatural Nonribosomal Peptides in Escherichia coli.

ABSTRACT: Peptides that are synthesized independently of the ribosome in plants, fungi, and bacteria can have clinically relevant anticancer, antihemochromatosis, and antiviral activities, among many other. Despite their natural origin, discovering new natural products is challenging, and there is a need to expand the chemical diversity that is accessible. In this work, we created a novel, compressed synthetic pathway for the heterologous expression and diversification of nonribosomal peptides (NRPs) based on homologs of siderophore pathways from Escherichia coli and Vibrio cholerae To enhance the likelihood of successful molecule production, we established a selective pressure via the iron-chelating properties of siderophores. By supplementing cells containing our synthetic pathway with different p

SUBMITTER: Cleto S 

PROVIDER: S-EPMC5635690 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature