A multiplex PCR mini-barcode assay to identify processed shark products in the global trade.

ABSTRACT: Protecting sharks from overexploitation has become global priority after widespread population declines have occurred. Tracking catches and trade on a species-specific basis has proven challenging, in part due to difficulties in identifying processed shark products such as fins, meat, and liver oil. This has hindered efforts to implement regulations aimed at promoting sustainable use of commercially important species and protection of imperiled species. Genetic approaches to identify shark products exist but are typically based on sequencing or amplifying large DNA regions and may fail to work on heavily processed products in which DNA is degraded. Here, we describe a novel multiplex PCR mini-barcode assay based on two short fragments of the cytochrome oxidase I (COI) gene. This assay can

SUBMITTER: Cardenosa D 

PROVIDER: S-EPMC5636071 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature