High-Fat Diet Changes Fungal Microbiomes and Interkingdom Relationships in the Murine Gut.

ABSTRACT: Dietary fat intake and shifts in gut bacterial community composition are associated with the development of obesity. To date, characterization of microbiota in lean versus obese subjects has been dominated by studies of gut bacteria. Fungi, recently shown to affect gut inflammation, have received little study for their role in obesity. We sought to determine the effects of high-fat diet on fungal and bacterial community structures in a mouse model using the internal transcribed spacer region 2 (ITS2) of fungal ribosomal DNA (rDNA) and the 16S rRNA genes of bacteria. Mice fed a high-fat diet had significantly different abundances of 19 bacterial and 6 fungal taxa than did mice fed standard chow, with high-fat diet causing similar magnitudes of change in overall fungal and bacterial microbio

SUBMITTER: Heisel T 

PROVIDER: S-EPMC5636226 | biostudies-literature | 2017 Sep-Oct

REPOSITORIES: biostudies-literature