A turn-on endoplasmic reticulum-targeted two-photon fluorescent probe for hydrogen sulfide and bio-imaging applications in living cells, tissues, and zebrafish.

ABSTRACT: As one of the important gas signal molecules, hydrogen sulfide (H₂S) is associated with many important physiological processes in living organisms. Organelles, especially endoplasmic reticulum (ER), play a crucial role in the cell metabolism. Accordingly, the detection of H₂S in the ER is of high interest. Toward this goal, we have described the development of the first ER-targeted fluorescent H₂S probe (Na-H ₂ S-ER). The new probe exhibited favorable features, such as a large turn-on fluorescence signal (45-fold fluorescence enhancement), high sensitivity and selectivity. The probe was successfully employed for imaging exogenous and endogenous H₂S in the living HeLa cells. Significantly, the new probe Na-H ₂ S-ER was employ

SUBMITTER: Tang Y 

PROVIDER: S-EPMC5636802 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature