Novel mechanism of regulation of the 5-lipoxygenase/leukotriene B₄ pathway by high-density lipoprotein in macrophages.

ABSTRACT: High-density lipoprotein (HDL) interacts with various cells, particularly macrophages, in functional cell-HDL interactions. Here, we found that HDL protein quality and lipid quality play critical roles in HDL functions. HDL fractions from healthy volunteers (HDL_Healthy) and patients with recurrent coronary atherosclerotic disease (HDL_CAD) were prepared. To analyse functional HDL-macrophage interactions, macrophages were co-incubated with each HDL, and lipid mediator production was assessed by liquid chromatography/mass spectrometry-based metabololipidomics. HDL_Healthy treatment attenuated the pro-inflammatory lipid mediator production, particularly that of leukotriene (LT) B₄, and this treatment enhanced lipoxin (LX) B₄ and resolvin (R

SUBMITTER: Tsuda S 

PROVIDER: S-EPMC5636875 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature