Characterization and antitumor activity of camptothecin from endophytic fungus Fusarium solani isolated from Camptotheca acuminate.

ABSTRACT:

Background

Camptothecin (CPT) is a potent drug against cancers, originally from plants. The endophytic fungi could produce the secondary metabolite same as the host and is used as medicine.

Objectives

The aim of this paper was to investigate an endophytic fungal CPT with anti-neoplastic activity.

Methods

Endophytic fungi were isolated from Camptotheca acuminata in China. CPT from strain S-019 was characterized by TLC, HPLC and EI-MS analysis. Anti-tumor activity of fungal CPT was detected by MTT and fluorescent dye methods using Vero and PC-3 cells.

Results

A total of 94 endophytic fungi strains were isolated from tissues of C. acuminata and 16 fungi strains displayed cytotoxic activity on Vero or PC3 cells. Of which, the fungal strain S-019, clas

SUBMITTER: Ran X 

PROVIDER: S-EPMC5637045 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature