Improved method for genomic DNA extraction for Opuntia Mill. (Cactaceae).

ABSTRACT:

Background

Genomic DNA extracted from species of Cactaceae is often contaminated with significant amounts of mucilage and pectin. Pectin is one of the main components of cellular walls, whereas mucilage is a complex polysaccharide with a ramified structure. Thus, pectin- and mucilage-free extraction of DNA is a key step for further downstream PCR-based analyses.

Results

We tested our DNA extraction method on cladode tissue (juvenile, adult, and herbaria exemplars) of 17 species of Opuntia Mill., which are characterized by a large quantity of pectin and mucilage.

Conclusion

We developed a method for the extraction of gDNA free of inhibitory compounds common in species of Opuntia Mill., such as pectin and mucilage. Compared to previously extraction protoco

SUBMITTER: Martinez-Gonzalez CR 

PROVIDER: S-EPMC5637266 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature