Identification of 76 novel B1 metallo-β-lactamases through large-scale screening of genomic and metagenomic data.

ABSTRACT:

Background

Metallo-β-lactamases are bacterial enzymes that provide resistance to carbapenems, the most potent class of antibiotics. These enzymes are commonly encoded on mobile genetic elements, which, together with their broad substrate spectrum and lack of clinically useful inhibitors, make them a particularly problematic class of antibiotic resistance determinants. We hypothesized that there is a large and unexplored reservoir of unknown metallo-β-lactamases, some of which may spread to pathogens, thereby threatening public health. The aim of this study was to identify novel metallo-β-lactamases of class B1, the most clinically important subclass of these enzymes.

Results

Based on a new computational method using an optimized hidden Markov model, we analyzed over 10,000 b

SUBMITTER: Berglund F 

PROVIDER: S-EPMC5637372 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature