Quantitative analysis of the tomato nuclear proteome during Phytophthora capsici infection unveils regulators of immunity.

ABSTRACT: Plant-pathogen interactions are complex associations driven by the interplay of host and microbe-encoded factors. With secreted pathogen proteins (effectors) and immune signalling components found in the plant nucleus, this compartment is a battleground where susceptibility is specified. We hypothesized that, by defining changes in the nuclear proteome during infection, we can pinpoint vital components required for immunity or susceptibility. We tested this hypothesis by documenting dynamic changes in the tomato (Solanum lycopersicum) nuclear proteome during infection by the oomycete pathogen Phytophthora capsici. We enriched nuclei from infected and noninfected tissues and quantitatively assessed changes in the nuclear proteome. We then tested the role of candidate regulators in immunity

SUBMITTER: Howden AJM 

PROVIDER: S-EPMC5637918 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature