Nipple aspirate fluid-A liquid biopsy for diagnosing breast health.

ABSTRACT:

Purpose

Nipple secretions are protein-rich and a potential source of breast cancer biomarkers for breast cancer screening. Previous studies of specific proteins have shown limited correlation with clinicopathological features. Our aim, in this pilot study, was to investigate the intra- and interpatient protein composition of nipple secretions and the implications for their use as liquid biopsies.

Experimental design

Matched pairs of nipple discharge/nipple aspirate fluid (NAF, n = 15) were characterized for physicochemical properties and SDS-PAGE. Four pairs were selected for semiquantitative proteomic profiling and trypsin-digested peptides analyzed using 2D-LC Orbitrap Fusion MS. The resulting data were subject to bioinformatics analysis and statistical evaluation for func

SUBMITTER: Shaheed SU 

PROVIDER: S-EPMC5638085 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature