Identifying direct contacts between protein complex subunits from their conditional dependence in proteomics datasets.

ABSTRACT: Determining the three dimensional arrangement of proteins in a complex is highly beneficial for uncovering mechanistic function and interpreting genetic variation in coding genes comprising protein complexes. There are several methods for determining co-complex interactions between proteins, among them co-fractionation / mass spectrometry (CF-MS), but it remains difficult to identify directly contacting subunits within a multi-protein complex. Correlation analysis of CF-MS profiles shows promise in detecting protein complexes as a whole but is limited in its ability to infer direct physical contacts among proteins in sub-complexes. To identify direct protein-protein contacts within human protein complexes we learn a sparse conditional dependency graph from approximately 3,000 CF-MS experim

SUBMITTER: Drew K 

PROVIDER: S-EPMC5638211 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature