Light transmission aggregometry using pre-coated microtiter plates and a Victor X5 plate reader.

ABSTRACT:

Background

Light transmission aggregometry (LTA) can be performed with microtiter plates (96-well LTA). When conducting LTA, an agonist is added to platelet-rich plasma and the sample is shaken for minutes after which absorbance readings are done. Platelet aggregation is detected as decrease in absorbance. However, the classical method is cumbersome and therefore microtiter plates can be used for concomitant testing of multiple samples. Furthermore, it would be convenient to prepare the plate in advance of platelet aggregation testing. Aim: The aim of the present study was to establish a simplified 96-well LTA protocol, where plates were pre-coated with agonists and stored at -80 C until use.

Results

We developed and validated a protocol for 96-well LTA using a Victor X5 pla

SUBMITTER: Vinholt PJ 

PROVIDER: S-EPMC5638243 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature