Sevoflurane protects rat brain endothelial barrier structure and function after hypoxia-reoxygenation injury.

ABSTRACT:

Background

After cerebral injury blood-brain barrier disruption significantly impairs brain homeostasis. Volatile anesthetics have been shown to be protective in ischemia-reperfusion injury scenarios. Their impact on brain endothelial cells after hypoxia-reoxygenation (H/R) has not yet been studied in detail.

Methods

Rat brain endothelial cells (RBE4) were exposed to severe hypoxia and reoxygenated in air in the presence or absence of sevoflurane. Changes in dextran permeability and architecture of the cellular junctional proteins ZO-1 and β-catenin were measured. To determine necrosis and apoptosis rate DNA content, LDH release and caspase activity were quantified. The role of vascular endothelial growth factor (VEGF) as an inflammatory mediator increasing vascular permeabi

SUBMITTER: Restin T 

PROVIDER: S-EPMC5638245 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature