DNA accumulation on ventilation system filters in university buildings in Singapore.

ABSTRACT:

Introduction

Biological particles deposit on air handling system filters as they process air. This study reports and interprets abundance and diversity information regarding biomass accumulation on ordinarily used filters acquired from several locations in a university environment.

Methods

DNA-based analysis was applied both to quantify (via DNA fluorometry and qPCR) and to characterize (via high-throughput sequencing) the microbial material on filters, which mainly processed recirculated indoor air. Results were interpreted in relation to building occupancy and ventilation system operational parameters.

Results

Based on accumulated biomass, average DNA concentrations per AHU filter surface area across nine indoor locations after twelve weeks of filter use were in the

SUBMITTER: Luhung I 

PROVIDER: S-EPMC5638523 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature