Vascular smooth muscle cells derived from inbred swine induced pluripotent stem cells for vascular tissue engineering.

ABSTRACT: Development of autologous tissue-engineered vascular constructs using vascular smooth muscle cells (VSMCs) derived from human induced pluripotent stem cells (iPSCs) holds great potential in treating patients with vascular disease. However, preclinical, large animal iPSC-based cellular and tissue models are required to evaluate safety and efficacy prior to clinical application. Herein, swine iPSC (siPSC) lines were established by introducing doxycycline-inducible reprogramming factors into fetal fibroblasts from a line of inbred Massachusetts General Hospital miniature swine that accept tissue and organ transplants without immunosuppression within the line. Highly enriched, functional VSMCs were derived from siPSCs based on addition of ascorbic acid and inactivation of reprogramming factor

SUBMITTER: Luo J 

PROVIDER: S-EPMC5638652 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature