Electropermeabilization of Inner and Outer Cell Membranes with Microsecond Pulsed Electric Fields: Quantitative Study with Calcium Ions.

ABSTRACT: Microsecond pulsed electric fields (μsPEF) permeabilize the plasma membrane (PM) and are widely used in research, medicine and biotechnology. For internal membranes permeabilization, nanosecond pulsed electric fields (nsPEF) are applied but this technology is complex to use. Here we report that the endoplasmic reticulum (ER) membrane can also be electropermeabilized by one 100 µs pulse without affecting the cell viability. Indeed, using Ca²⁺ as a permeabilization marker, we observed cytosolic Ca²⁺ peaks in two different cell types after one 100 µs pulse in a medium without Ca²⁺. Thapsigargin abolished these Ca²⁺ peaks demonstrating that the calcium is released from the ER. Moreover, IP3R and RyR inhibitors did not modify these peaks showing that

SUBMITTER: Hanna H 

PROVIDER: S-EPMC5638809 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature