The folding, stability and function of lactose permease differ in their dependence on bilayer lipid composition.

ABSTRACT: Lipids play key roles in Biology. Mechanical properties of the lipid bilayer influence their neighbouring membrane proteins, however it is unknown whether different membrane protein properties have the same dependence on membrane mechanics, or whether mechanics are tuned to specific protein processes of the protein. We study the influence of lipid lateral pressure and electrostatic effects on the in vitro reconstitution, folding, stability and function of a representative of the ubiquitous major facilitator transporter superfamily, lactose permease. Increasing the outward chain lateral pressure in the bilayer, through addition of lamellar phosphatidylethanolamine lipids, lowers lactose permease folding and reconstitution yields but stabilises the folded state. The presence of phosphatidyle

SUBMITTER: Findlay HE 

PROVIDER: S-EPMC5638818 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature