Highly efficient cellular cloning using Ferro-core Micropallet Arrays.

ABSTRACT: Advancing knowledge of biological mechanisms has come to depend upon genetic manipulation of cells and organisms, relying upon cellular cloning methods that remain unchanged for decades, are labor and time intensive, often taking many months to come to fruition. Thus, there is a pressing need for more efficient processes. We have adapted a newly developed micropallet array platform, termed the "ferro-core micropallet array", to dramatically improve and accelerate the process of isolating clonal populations of adherent cells from heterogeneous mixtures retaining the flexibility of employing a wide range of cytometric parameters for identifying colonies and cells of interest. Using transfected (retroviral oncogene or fluorescent reporter construct) rat 208 F cells, we demonstrated the capaci

SUBMITTER: Westerhof TM 

PROVIDER: S-EPMC5638909 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature