Translesion synthesis DNA polymerase η exhibits a specific RNA extension activity and a transcription-associated function.

ABSTRACT: Polymerase eta (Polη) is a low fidelity translesion synthesis DNA polymerase that rescues damage-stalled replication by inserting deoxy-ribonucleotides opposite DNA damage sites resulting in error-free or mutagenic damage bypass. In this study we identify a new specific RNA extension activity of Polη of Saccharomyces cerevisiae. We show that Polη is able to extend RNA primers in the presence of ribonucleotides (rNTPs), and that these reactions are an order of magnitude more efficient than the misinsertion of rNTPs into DNA. Moreover, during RNA extension Polη performs error-free bypass of the 8-oxoguanine and thymine dimer DNA lesions, though with a 10³ and 10²-fold lower efficiency, respectively, than it synthesizes opposite undamaged nucleotides. Furthermore, in viv

SUBMITTER: Gali VK 

PROVIDER: S-EPMC5638924 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature