Multiple Approaches Detect the Presence of Fungi in Human Breastmilk Samples from Healthy Mothers.

ABSTRACT: Human breastmilk contains a variety of bacteria that are transmitted to the infant and have been suggested to contribute to gut microbiota development and immune maturation. However, the characterization of fungal organisms in milk from healthy mothers is currently unknown although their presence has been reported in the infant gut and also in milk from other mammals. Breastmilk samples from healthy lactating mothers (n = 65) within 1 month after birth were analyzed. Fungal presence was assessed by different techniques, including microscopy, growth and identification of cultured isolates, fungal load estimation by qPCR, and fungal composition using 28S rRNA gene high-throughput sequencing. In addition, milk macronutrients and human somatic cells were quantified by spectrophotometry and cyt

SUBMITTER: Boix-Amoros A 

PROVIDER: S-EPMC5638952 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature