Cloning and sequence analysis of hyaluronoglucosaminidase (nagH) gene of Clostridium chauvoei.

ABSTRACT:

Aim

Blackleg disease is caused by Clostridium chauvoei in ruminants. Although virulence factors such as C. chauvoei toxin A, sialidase, and flagellin are well characterized, hyaluronidases of C. chauvoei are not characterized. The present study was aimed at cloning and sequence analysis of hyaluronoglucosaminidase (nagH) gene of C. chauvoei.

Materials and methods

C. chauvoei strain ATCC 10092 was grown in ATCC 2107 media and confirmed by polymerase chain reaction (PCR) using the primers specific for 16-23S rDNA spacer region. nagH gene of C. chauvoei was amplified and cloned into pRham-SUMO vector and transformed into Escherichia cloni 10G cells. The construct was then transformed into E. cloni cells. Colo

SUBMITTER: Dangi SK 

PROVIDER: S-EPMC5639109 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature