Single-nucleus Hi-C reveals unique chromatin reorganization at oocyte-to-zygote transition.

ABSTRACT: Chromatin is reprogrammed after fertilization to produce a totipotent zygote with the potential to generate a new organism. The maternal genome inherited from the oocyte and the paternal genome provided by sperm coexist as separate haploid nuclei in the zygote. How these two epigenetically distinct genomes are spatially organized is poorly understood. Existing chromosome conformation capture-based methods are not applicable to oocytes and zygotes owing to a paucity of material. To study three-dimensional chromatin organization in rare cell types, we developed a single-nucleus Hi-C (high-resolution chromosome conformation capture) protocol that provides greater than tenfold more contacts per cell than the previous method. Here we show that chromatin architecture is uniquely reorganized duri

SUBMITTER: Flyamer IM 

PROVIDER: S-EPMC5639698 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature