Molecular characterization of human osteoblast-derived extracellular vesicle mRNA using next-generation sequencing.

ABSTRACT: Extracellular vesicles (EVs) are membrane-bound intercellular communication vehicles that transport proteins, lipids and nucleic acids with regulatory capacity between cells. RNA profiling using microarrays and sequencing technologies has revolutionized the discovery of EV-RNA content, which is crucial to understand the molecular mechanism of EV function. Recent studies have indicated that EVs are enriched with specific RNAs compared to the originating cells suggestive of an active sorting mechanism. Here, we present the comparative transcriptome analysis of human osteoblasts and their corresponding EVs using next-generation sequencing. We demonstrate that osteoblast-EVs are specifically depleted of cellular mRNAs that encode proteins involved in basic cellular activities, such as cytoskel

SUBMITTER: Morhayim J 

PROVIDER: S-EPMC5639703 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature