Neutrophil Activation of Endothelial Cell-Expressed TRPM2 Mediates Transendothelial Neutrophil Migration and Vascular Injury.

ABSTRACT:

Rationale

TRPM2 (transient receptor potential melastatin-2) expressed in endothelial cells (ECs) is a cation channel mediating Ca²⁺ entry in response to intracellular generation of adenosine diphosphoribose-the TRPM2 ligand.

Objective

Because polymorphonuclear neutrophils (PMN) interaction with ECs generates reactive oxygen species, we addressed the possible role of TRPM2 expressed in ECs in the mechanism of transendothelial migration of PMNs.

Methods and results

We observed defective PMN transmigration in response to lipopolysaccharide challenge in adult mice in which the EC expressed TRPM2 is conditionally deleted (Trpm2^iΔEC ). PMN interaction with ECs induced the entry of Ca²⁺ in ECs via the EC-expressed TRPM2. Prevent

SUBMITTER: Mittal M 

PROVIDER: S-EPMC5640489 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature