Absolute quantification of proteins in the fatty acid biosynthetic pathway using protein standard absolute quantification.

ABSTRACT: With worldwide attention on renewable energy and climate change, metabolic engineering of the fatty acid biosynthetic pathway has become an active area of research, with a view to enhance production of biofuels. Indeed, this pathway has already been extensively studied in Escherichia coli. Nevertheless, little is known about the absolute abundance of the enzymes involved, information that may be valuable for engineering, such as the optimal molar ratios of different proteins. In this study, we use protein standard absolute quantification (PSAQ) to measure the absolute abundance of proteins that catalyze fatty acid biosynthesis in E. coli. In addition, the changes of protein abundance were analyzed by comparing the differences between high-yield and the background strain. Our

SUBMITTER: Tao H 

PROVIDER: S-EPMC5640790 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature