Sequence-specific detection of different strains of LCMV in a single sample using tentacle probes.

ABSTRACT:

Background

Virus infections often result in quasispecies of viral strains that can have dramatic impacts on disease outcomes. However, sequencing of viruses to determine strain composition is time consuming and often cost-prohibitive. Rapid, cost-effective methods are needed for accurate measurement of virus diversity to understand virus evolution and can be useful for experimental systems.

Methods

We have developed a novel molecular method for sequence-specific detection of RNA virus genetic variants called Tentacle Probes. The probes are modified molecular beacons that have dramatically improved false positive rates and specificity in routine qPCR. To validate this approach, we have designed Tentacle Probes for two different strains of Lymphocytic Choriomeningitis Virus (L

SUBMITTER: Franco LS 

PROVIDER: S-EPMC5640925 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature