Cellobiohydrolase B of Aspergillus niger over-expressed in Pichia pastoris stimulates hydrolysis of oil palm empty fruit bunches.

ABSTRACT:

Background

Aspergillus niger, along with many other lignocellulolytic fungi, has been widely used as a commercial workhorse for cellulase production. A fungal cellulase system generally includes three major classes of enzymes i.e., β-glucosidases, endoglucanases and cellobiohydrolases. Cellobiohydrolases (CBH) are vital to the degradation of crystalline cellulose present in lignocellulosic biomass. However, A. niger naturally secretes low levels of CBH. Hence, recombinant production of A. niger CBH is desirable to increase CBH production yield and also to allow biochemical characterisation of the recombinant CBH from A. niger.

Methods

In this study, the gene encoding a cellobiohydrolase B (cbhB) from A. niger ATCC 10574 was cloned an

SUBMITTER: Woon JS 

PROVIDER: S-EPMC5641429 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature