Home Alone: Elimination of All but One Alternative Sigma Factor in Listeria monocytogenes Allows Prediction of New Roles for σ^B.

ABSTRACT: Among Listeria monocytogenes' four alternative σ factors, σ^B controls the largest regulon. As σ^B-dependent transcription of some genes may be masked by overlaps among regulons, and as some σ^B-dependent genes are expressed only under very specific conditions, we hypothesized that the σ^B regulon is not yet fully defined. To further extend our understanding of the σ^B regulon, we used RNA-seq to identify σ^B-dependent genes in an L. monocytogenes strain that expresses σ^B following rhamnose induction, and in which genes encoding the other alternative sigma factors have been deleted. Analysis of RNA-seq data with multiple bioinformatics approaches, including a sliding window method that detects differential

SUBMITTER: Liu Y 

PROVIDER: S-EPMC5641562 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature