Carbon Allocation in Rhodococcus jostii RHA1 in Response to Disruption and Overexpression of nlpR Regulatory Gene, Based on ¹³C-labeling Analysis.

ABSTRACT: Nitrogen lipid regulator (NlpR) is a pleiotropic regulator that positively controls genes associated with both nitrogen and lipid metabolism in the oleaginous bacterium Rhodococcus jostii RHA1. In this study, we investigated the effect of nlpR disruption and overexpression on the assimilation of ¹³C-labeled glucose as carbon source, during cultivation of cells under nitrogen-limiting and nitrogen-rich conditions, respectively. Label incorporation into the total lipid extract (TLE) fraction was about 30% lower in the mutant strain in comparison with the wild type strain under low-nitrogen conditions. Moreover, a higher ¹³C abundance (∼60%) into the extracellular polymeric substance fraction was observed in the mutant strain. nlpR disruption also pr

SUBMITTER: Hernandez MA 

PROVIDER: S-EPMC5641563 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature