Intestinal SIRT3 overexpression in mice improves whole body glucose homeostasis independent of body weight.

ABSTRACT:

Objective

Intestinal metabolism might play a greater role in regulating whole body metabolism than previously believed. We aimed to enhance enterocyte metabolism in mice and investigate if it plays a role in diet-induced obesity (DIO) and its comorbidities.

Methods

Using the cre-loxP system, we overexpressed the mitochondrial NAD⁺ dependent protein deacetylase SIRT3 in enterocytes of mice (iSIRT3 mice). We chronically fed iSIRT3 mice and floxed-SIRT3 control (S3fl) mice a low-fat, control diet (CD) or a high-fat diet (HFD) and then phenotyped the mice.

Results

There were no genotype differences in any of the parameters tested when the mice were fed CD. Also, iSIRT3 mice were equally susceptible to the development of DIO as S3fl mice when fed HFD. They were,

SUBMITTER: Ramachandran D 

PROVIDER: S-EPMC5641632 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature