Identification and characterization of Vibrio vulnificus plpA encoding a phospholipase A₂ essential for pathogenesis.

ABSTRACT: The marine bacterium Vibrio vulnificus causes food-borne diseases, which may lead to life-threatening septicemia in some individuals. Therefore, identifying virulence factors in V. vulnificus is of high priority. We performed a transcriptome analysis on V. vulnificus after infection of human intestinal HT29-methotrexate cells and found induction of plpA, encoding a putative phospholipase, VvPlpA. Bioinformatics, biochemical, and genetic analyses demonstrated that VvPlpA is a phospholipase A₂ secreted in a type II secretion system-dependent manner. Compared with the wild type, the plpA mutant exhibited reduced mortality, systemic infection, and inflammation in mice as well as low cytotoxicity toward the human epithelial INT-407 cel

SUBMITTER: Jang KK 

PROVIDER: S-EPMC5641866 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature