Efficient reduction of CO₂ by the molybdenum-containing formate dehydrogenase from Cupriavidus necator (Ralstonia eutropha).

ABSTRACT: The ability of the FdsABG formate dehydrogenase from Cupriavidus necator (formerly known as Ralstonia eutropha) to catalyze the reverse of the physiological reaction, the reduction of CO₂ to formate utilizing NADH as electron donor, has been investigated. Contrary to previous studies of this enzyme, we demonstrate that it is in fact effective in catalyzing the reverse reaction with a k_cat of 11 ± 0.4 s^-1 We also quantify the stoichiometric accumulation of formic acid as the product of the reaction and demonstrate that the observed kinetic parameters for catalysis in the forward and reverse reactions are thermodynamically consistent, complying with the expected Haldane relationships. Finally, we demonstrate the reaction conditions necess

SUBMITTER: Yu X 

PROVIDER: S-EPMC5641872 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature