Intestinal Enteroendocrine Lineage Cells Possess Homeostatic and Injury-Inducible Stem Cell Activity.

ABSTRACT: Several cell populations have been reported to possess intestinal stem cell (ISC) activity during homeostasis and injury-induced regeneration. Here, we explored inter-relationships between putative mouse ISC populations by comparative RNA-sequencing (RNA-seq). The transcriptomes of multiple cycling ISC populations closely resembled Lgr5⁺ ISCs, the most well-defined ISC pool, but Bmi1-GFP⁺ cells were distinct and enriched for enteroendocrine (EE) markers, including Prox1. Prox1-GFP⁺ cells exhibited sustained clonogenic growth in vitro, and lineage-tracing of Prox1⁺ cells revealed long-lived clones during homeostasis and after radiation-induced injury in vivo. Single-cell mRNA-seq revealed two subsets of Prox1-GFP⁺ cells, one of which re

SUBMITTER: Yan KS 

PROVIDER: S-EPMC5642297 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature