RNAi-mediated knockdown of Parp1 does not improve the development of female cloned mouse embryos.

ABSTRACT: Somatic cell nuclear transfer is an important technique for life science research, but its efficiency is still extremely low, and most genes that are important during early development, such as X chromosome-linked genes, are not appropriately expressed during this process. Poly (ADP-ribose) polymerase (PARP) is an enzyme that transfers ADP ribose clusters to target proteins. PARP family members such as PARP1 participate in cellular signalling pathways through poly (ADP-ribosylation) (PARylation), which ultimately promotes changes in chromatin structure, gene expression, and the localization and activity of proteins that mediate signalling responses. PARP1 is associated with X chromosome inactivation (Xi). Here, we showed that abnormal Xi occurs in somatic cell nuclear transfer (NT) blastoc

SUBMITTER: Bai GY 

PROVIDER: S-EPMC5642522 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature