Ontology highlight
ABSTRACT:
SUBMITTER: Schwartz ML
PROVIDER: S-EPMC5643095 | biostudies-literature | 2017 Sep
REPOSITORIES: biostudies-literature
eLife 20170919
Zippering of SNARE complexes spanning docked membranes is essential for most intracellular fusion events. Here, we explore how SNARE regulators operate on discrete zippering states. The formation of a metastable trans-complex, catalyzed by HOPS and its SM subunit Vps33, is followed by subsequent zippering transitions that increase the probability of fusion. Operating independently of Sec18 (NSF) catalysis, Sec17 (α-SNAP) either inhibits or stimulates SNARE-mediated fusion. If HOPS or Vps33 are a ...[more]