A quantitative comparison of cytosolic delivery via different protein uptake systems.

ABSTRACT: Over many years, a variety of delivery systems have been investigated that have the capacity to shuttle macromolecular cargoes, especially proteins, into the cytosol. Due to the lack of an objective way to quantify cytosolic delivery, relative delivery efficiencies of the various transport systems have remained unclear. Here, we demonstrate the use of the biotin ligase assay for a quantitative comparison of protein transport to the cytosol via cell-penetrating peptides, supercharged proteins and bacterial toxins in four different cell lines. The data illustrate large differences in both the total cellular internalization, which denotes any intracellular location including endosomes, and in the cytosolic uptake of the transport systems, with little correlation between the two. Also, we foun

SUBMITTER: Verdurmen WPR 

PROVIDER: S-EPMC5643320 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature