Ontology highlight
ABSTRACT:
SUBMITTER: Oakes T
PROVIDER: S-EPMC5643411 | biostudies-literature | 2017
REPOSITORIES: biostudies-literature
Oakes Theres T Heather James M JM Best Katharine K Byng-Maddick Rachel R Husovsky Connor C Ismail Mazlina M Joshi Kroopa K Maxwell Gavin G Noursadeghi Mahdad M Riddell Natalie N Ruehl Tabea T Turner Carolin T CT Uddin Imran I Chain Benny B
Frontiers in immunology 20171012
The T cell receptor (TCR) repertoire can provide a personalized biomarker for infectious and non-infectious diseases. We describe a protocol for amplifying, sequencing, and analyzing TCRs which is robust, sensitive, and versatile. The key experimental step is ligation of a single-stranded oligonucleotide to the 3' end of the TCR cDNA. This allows amplification of all possible rearrangements using a single set of primers per locus. It also introduces a unique molecular identifier to label each st ...[more]