RNA-Seq transcriptome profiling of mouse oocytes after in vitro maturation and/or vitrification.

ABSTRACT: In vitro maturation (IVM) and vitrification have been widely used to prepare oocytes before fertilization; however, potential effects of these procedures, such as expression profile changes, are poorly understood. In this study, mouse oocytes were divided into four groups and subjected to combinations of in vitro maturation and/or vitrification treatments. RNA-seq and in silico pathway analysis were used to identify differentially expressed genes (DEGs) that may be involved in oocyte viability after in vitro maturation and/or vitrification. Our results showed that 1) 69 genes were differentially expressed after IVM, 66 of which were up-regulated. Atp5e and Atp5o were enriched in the most significant gene ontology term "mitochondrial membrane part"; thus, these genes may be promising candid

SUBMITTER: Gao L 

PROVIDER: S-EPMC5643491 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature