Biochemical Investigation of Rv3404c from Mycobacterium tuberculosis.

ABSTRACT: The causative agent of tuberculosis, Mycobacterium tuberculosis, is a bacterium with a complex cell wall and a complicated life cycle. The genome of M. tuberculosis contains well over 4000 genes thought to encode proteins. One of these codes for a putative enzyme referred to as Rv3404c, which has attracted research attention as a potential virulence factor for over 12 years. Here we demonstrate that Rv3404c functions as a sugar N-formyltransferase that converts dTDP-4-amino-4,6-dideoxyglucose into dTDP-4-formamido-4,6-dideoxyglucose using N¹⁰-formyltetrahydrofolate as the carbon source. Kinetic analyses demonstrate that Rv3404c displays a significant catalytic efficiency of 1.1 × 10⁴ M^-1 s^-1. In addition, we report the X-ray structure of a ternar

SUBMITTER: Dunsirn MM 

PROVIDER: S-EPMC5644030 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature