Mechanistic Insight into Sonoporation with Ultrasound-Stimulated Polymer Microbubbles.

ABSTRACT: Sonoporation is emerging as a feasible, non-viral gene delivery platform for the treatment of cardiovascular disease and cancer. Despite promising results, this approach remains less efficient than viral methods. The objective of this work is to help substantiate the merit of polymeric microbubble sonoporation as a non-viral, localized cell permeation and payload delivery strategy by taking a ground-up approach to elucidating the fundamental mechanisms at play. In this study, we apply simultaneous microscopy of polymeric microbubble sonoporation over its intrinsic biophysical timescales-with sub-microsecond resolution to examine microbubble cavitation and millisecond resolution over several minutes to examine local macromolecule uptake through enhanced endothelial cell membrane permeabilit

SUBMITTER: Helfield BL 

PROVIDER: S-EPMC5644032 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature