De novo assembly of genomes from long sequence reads reveals uncharted territories of Propionibacterium freudenreichii.

ABSTRACT:

Background

Propionibacterium freudenreichii is an industrially important bacterium granted the Generally Recognized as Safe (the GRAS) status, due to its long safe use in food bioprocesses. Despite the recognized role in the food industry and in the production of vitamin B12, as well as its documented health-promoting potential, P. freudenreichii remained poorly characterised at the genomic level. At present, only three complete genome sequences are available for the species.

Results

We used the PacBio RS II sequencing platform to generate complete genomes of 20 P. freudenreichii strains and compared them in detail. Comparative analyses revealed both sequence conservation and genome organisational diversity among the strains. Assembly from long reads resulted in the discover

SUBMITTER: Deptula P 

PROVIDER: S-EPMC5644110 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature