High-Throughput Inertial Focusing of Micrometer- and Sub-Micrometer-Sized Particles Separation.

ABSTRACT: The ability to study individual bacteria or subcellular organelles using inertial microfluidics is still nascent. This is due, in no small part, to the significant challenges associated with concentrating and separating specific sizes of micrometer and sub-micrometer bioparticles in a microfluidic format. In this study, using a rigid polymeric microfluidic network with optimized microchannel geometry dimensions, it is demonstrated that 2 µm, and even sub-micrometer, particles can be continuously and accurately focused to stable equilibrium positions. Suspensions have been processed at flow rates up to 1400 µL min^-1 in an ultrashort 4 mm working channel length. A wide range of suspension concentrations-from 0.01 to 1 v/v%-have been systematically investigated, with yields greater

SUBMITTER: Wang L 

PROVIDER: S-EPMC5644225 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature