Novel Application of Magnetic Protein: Convenient One-Step Purification and Immobilization of Proteins.

ABSTRACT: Recently, a magnetic protein was discovered, and a multimeric magnetosensing complex was validated, which may form the basis of magnetoreception. In this study, the magnetic protein was firstly used in biotechnology application, and a novel convenient one-step purification and immobilization method was established. A universal vector and three linker patterns were developed for fusion expression of magnetic protein and target protein. The magnetic protein was absorbed by iron beads, followed by target protein aggregation, purification, and immobilization. GFP, employed as a reporter protein, was successfully purified from cell lysate. Subsequently, three enzymes (lipase, α-L-arabinofuranosidase, pullulanase) with different molecular sizes testified the versatility of this magnetic-based ap

SUBMITTER: Jiang M 

PROVIDER: S-EPMC5645317 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature