Glycosylation-related gene expression in HT29-MTX-E12 cells upon infection by Helicobacter pylori.

ABSTRACT:

Aim

To identify glycosylation-related genes in the HT29 derivative cell line, HT29-MTX-E12, showing differential expression on infection with Helicobacter pylori (H. pylori).

Methods

Polarised HT29-MTX-E12 cells were infected for 24 h with H. pylori strain 26695. After infection RNA was isolated from both infected and non-infected host cells. Sufficient infections were carried out to provide triplicate samples for microarray analysis and for qRT-PCR analysis. RNA was isolated and hybridised to Affymetrix arrays. Analysis of microarray data identified genes significantly differentially expressed upon infection. Genes were grouped into gene ontology functional categories. Selected genes associated with host glycan structure (glycosyltransferases, hydrolase

SUBMITTER: Cairns MT 

PROVIDER: S-EPMC5645615 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature