Thermodynamics and kinetics of DNA nanotube polymerization from single-filament measurements.

ABSTRACT: DNA nanotubes provide a programmable architecture for molecular self-assembly and can serve as model systems for one-dimensional biomolecular assemblies. While a variety of DNA nanotubes have been synthesized and employed as models for natural biopolymers, an extensive investigation of DNA nanotube kinetics and thermodynamics has been lacking. Using total internal reflection microscopy, DNA nanotube polymerization was monitored in real time at the single filament level over a wide range of free monomer concentrations and temperatures. The measured polymerization rates were subjected to a global nonlinear fit based on polymerization theory in order to simultaneously extract kinetic and thermodynamic parameters. For the DNA nanotubes used in this study, the association rate constant is (5.99

SUBMITTER: Hariadi RF 

PROVIDER: S-EPMC5645730 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature