Enrichment allows identification of diverse, rare elements in metagenomic resistome-virulome sequencing.

ABSTRACT:

Background

Shotgun metagenomic sequencing is increasingly utilized as a tool to evaluate ecological-level dynamics of antimicrobial resistance and virulence, in conjunction with microbiome analysis. Interest in use of this method for environmental surveillance of antimicrobial resistance and pathogenic microorganisms is also increasing. In published metagenomic datasets, the total of all resistance- and virulence-related sequences accounts for < 1% of all sequenced DNA, leading to limitations in detection of low-abundance resistome-virulome elements. This study describes the extent and composition of the low-abundance portion of the resistome-virulome, using a bait-capture and enrichment system that incorporates unique molecular indices to count DNA molecules and correct for enrich

SUBMITTER: Noyes NR 

PROVIDER: S-EPMC5645900 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature