Assessing biological and technological variability in protein levels measured in pre-diagnostic plasma samples of women with breast cancer.

ABSTRACT:

Background

Quantitative proteomics allows for the discovery and functional investigation of blood-based pre-diagnostic biomarkers for early cancer detection. However, a major limitation of proteomic investigations in biomarker studies remains the biological and technical variability in the analysis of complex clinical samples. Moreover, unlike 'omics analogues such as genomics and transcriptomics, proteomics has yet to achieve reproducibility and long-term stability on a unified technological platform. Few studies have thoroughly investigated protein variability in pre-diagnostic samples of cancer patients across multiple platforms.

Methods

We obtained ten blood plasma "case" samples collected up to 2 years prior to breast cancer diagnosis. Each case sample was paired with a

SUBMITTER: Yeh CY 

PROVIDER: S-EPMC5645980 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature