Establishment and characterization of novel epithelial-like cell lines derived from human periodontal ligament tissue in vitro.

ABSTRACT: In this study, novel human-derived epithelial-like cells (hEPLCs) lines were established from periodontal ligament (PDL) tissues, which were composed of a variety of cell types and exhibited complex cellular activities. To elucidate the putative features distinguishing these from epithelial rest of Malassez (ERM), we characterized hEPLCs based on cell lineage markers and tight junction protein expression. The aim of this study was, therefore, to establish and characterize hEPLCs lines from PDL tissues. The hEPLCs were isolated from PDL of third molar teeth. Cellular morphology and cell organelles were observed thoroughly. The characteristics of epithelial-endothelial-mesenchymal-like cells were compared in several markers by gene expression and immunofluorescence, to ERM and human umbilica

SUBMITTER: Tansriratanawong K 

PROVIDER: S-EPMC5646140 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature