Global Assessment of Dengue Virus-Specific CD4⁺ T Cell Responses in Dengue-Endemic Areas.

ABSTRACT:

Background

Dengue is a major public health problem worldwide. Assessment of adaptive immunity is important to understanding immunopathology and to define correlates of protection against dengue virus (DENV). To enable global assessment of CD4⁺ T cell responses, we mapped HLA-DRB1-restricted DENV-specific CD4⁺ T cell epitopes in individuals previously exposed to DENV in the general population of the dengue-endemic region of Managua, Nicaragua.

Methods

HLA class II epitopes in the population of Managua were identified by an in vitro IFNγ ELISPOT assay. CD4⁺ T cells purified by magnetic bead negative selection were stimulated with HLA-matched epitope pools in the presence of autologous antigen-presenting cells, followed by pool deconvolut

SUBMITTER: Grifoni A 

PROVIDER: S-EPMC5646259 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature