Osteopontin adsorption to Gram-positive cells reduces adhesion forces and attachment to surfaces under flow.

ABSTRACT: The bovine milk protein osteopontin (OPN) may be an efficient means to prevent bacterial adhesion to dental tissues and control biofilm formation. This study sought to determine to what extent OPN impacts adhesion forces and surface attachment of different bacterial strains involved in dental caries or medical device-related infections. It further investigated if OPN's effect on adhesion is caused by blocking the accessibility of glycoconjugates on bacterial surfaces. Bacterial adhesion was determined in a shear-controlled flow cell system in the presence of different concentrations of OPN, and interaction forces of single bacteria were quantified using single-cell force spectroscopy before and after OPN exposure. Moreover, the study investigated OPN's effect on the accessibility of cell s

SUBMITTER: Kristensen MF 

PROVIDER: S-EPMC5646589 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature